Manufacture of a human mesenchymal stem cell population using an automated cell culture platform
نویسندگان
چکیده
منابع مشابه
Manufacture of a human mesenchymal stem cell population using an automated cell culture platform.
Tissue engineering and regenerative medicine are rapidly developing fields that use cells or cell-based constructs as therapeutic products for a wide range of clinical applications. Efforts to commercialise these therapies are driving a need for capable, scaleable, manufacturing technologies to ensure therapies are able to meet regulatory requirements and are economically viable at industrial s...
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Objective Articular cartilage as an avascular skeletal tissue possesses limited capacity to heal. On the other hand, it is believed that the regeneration capacity of each tissue is largely related to its stem cell contents. Little is known about the presence of mesenchymal stem cells in articular cartilage tissue. This subject is investigated in the present study. Materials and Methods Artic...
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Human mesenchymal stem cells (hMSCs), which also called mesenchymal stromal cells, are multipotent stem cell. Human MSCs typically are positive for the surface markers CD44, CD73, CD90, CD105, CD106, and also negative for hematopoietic markers CD34 and CD45.These cells can be isolated from postnatal bone marrow, adipose tissue, placenta, and scalp tissue, as well as from various fetal tissues. ...
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The importance of providing a physiologically relevant environment for cell culture is well recognized. The combination of proper environmental cues which are provided in vivo by the bloodstream and extracellular matrix must be reproduced to properly examine cell response in vitro, and cannot be recapitulated using traditional culture on polystyrene. Here, we have developed a device, the dynami...
متن کاملmesenchymal stem cell purification from the articular cartilage cell culture
objective articular cartilage as an avascular skeletal tissue possesses limited capacity to heal. on the other hand, it is believed that the regeneration capacity of each tissue is largely related to its stem cell contents. little is known about the presence of mesenchymal stem cells in articular cartilage tissue. this subject is investigated in the present study. materials and methods articula...
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ژورنال
عنوان ژورنال: Cytotechnology
سال: 2007
ISSN: 0920-9069,1573-0778
DOI: 10.1007/s10616-007-9091-2